Production of N-acetyl chitooligosaccharide by novel Streptomyces chilikensis strain RC1830 and its evaluation for anti-radical, anti-inflammatory, anti-proliferative and cell migration potential

Abstract

The present study focuses on the preparation of N-acetyl chitooligosaccharides (N-AcCOS) by microbial degradation of colloidal chitin using novel Streptomyces chilikensis strain RC1830 instead of using chitinolytic enzymes extracted from microorganisms as reported earlier. Statistical optimization of the factors such as pH, media, carbon source, nitrogen source, salt concentration, surfactants and metal ions on production of N-AcCOS by the strain RC1830 at shake flask level and subsequent validation of the optimized model at bench scale bioreactor level were conducted. The product obtained was characterized by Fourier Transformed Infrared Spectroscopy (FTIR) and Electrospray Ionization Mass Spectrometry (ESI-MS). N-AcCOS obtained, was evaluated for in vitro anti-radical, anti-inflammatory, anti-proliferative activity and its role in cell migration. The results showed that the N-AcCOS extract has free radical scavenging and anti-inflammatory activities and down regulation of proliferation of human breast cancer cells in a dose dependent manner. Cell migration assay showed that N-AcCOS extract promoted effective migration of HEK293 cell at a concentration of 750 μg/mL and 1000 μg/mL.