Production of Mycological Media using Local Raw Inedible Materials

Abstract

The use of local inedible raw materials in the formulation of mycological laboratory medium which is cost effective was carried out using brewer’s spent grain (BSG) samples gotten from Intafact Breweries Onitsha, Anambra state. The BSG was mixed with 5g of cow blood, 20g of Ede mmuo (Caladium bicolor), 5g of Fiofio, and 20g of agar agar as a gelling agent. The other media was prepared with the same materials but without agar agar. The formulated media was inoculated with Aspergillus niger as the test fungus. The two different media were compared for gowth diameter. The fungal organism grew faster on agar agar medium with wider colony diameter. On agar-agar medium, the length on the first day for the four plates were 1cm each. Second day for the four plates include; plate 1(15.0mm), plate 2(20.0mm), plate 3(10mm), and plate 4(15mm). Third day include; plate 1(30mm), plate 2(40mm), plate 3(32mm), and plate 4(35mm). Fourth day include; plate 1(60mm), plate 2(50mm), plate 3(35mm), and plate 4(60mm). Non agar-agar medium, the length on the first day for the four plates were 3mm each. Second day for the four plates include; plate 1(10.0mm), plate 2(11.0mm), plate 3(11.0mm), and plate 4(10.0mm). Third day include; plate 1(12.0mm), plate 2(14.0mm), plate 3(13.0mm), and plate 4(13.0mm). Fourth day include; plate 1(15.0mm), plate 2(16.0mm), plate 3(14.0mm), and plate 4(15.0mm).