Abstract
Background Monoclonal antibodies (Mabs) have been recently used for the treatment of virtually every debilitating disease. Packed-bed bioreactors have been used for the cultivation and production of a wide range of cell lines and biologics including MAbs. The principle behind a Packed-bed bioreactor is that the cells are being immobilized within a suitable stationary matrix which is represented by the bed. Packed-bed bioreactors also have the advantage of being capable of generating high cell densities with a low concentration of free cells in suspension; hence, simplifying downstream processing. The present work was designed to compare between the cultivation of hybridoma cells as well as the production of OKT3 MAb in free and immobilized culture conditions.
Highlights
Monoclonal antibodies (Mabs) have been recently used for the treatment of virtually every debilitating disease
Determination of MAb specificity Secondary fluorescence antibodies bounded to the produced antibody which in turn is bound to CD3 positive lymphocytes (T-cells) showed a percentage of CD3 positive lymphocytes of 76.68%
Continuous production of MAb by the cultivation of hybridoma cells in Basket spinner In this work two Basket Spinners were used in parallel, one of them was packed with 5 gm of Fibra-Cel disks (Figure 1), and the other was used as control without packing
Summary
Monoclonal antibodies (Mabs) have been recently used for the treatment of virtually every debilitating disease. Packed-bed bioreactors have been used for the cultivation and production of a wide range of cell lines and biologics including MAbs. The principle behind a Packed-bed bioreactor is that the cells are being immobilized within a suitable stationary matrix which is represented by the bed. Packed-bed bioreactors have the advantage of being capable of generating high cell densities with a low concentration of free cells in suspension; simplifying downstream processing. The present work was designed to compare between the cultivation of hybridoma cells as well as the production of OKT3 MAb in free and immobilized culture conditions
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