Abstract
Mouse-mouse hybridoma cells producing IgG against α-amylase were cultivated with a radial-flow bioreactor (RA-400), and continuous production of monoclonal antibodies was tested. After inoculation, the cells grew exponentially, and both the fresh medium feed rate and glucose consumption rate also increased exponentially. After 7 days from the start of cultivation, the fresh medium feed rate attained almost a steady state, the final feed rate being 18 1/day. The IgG concentration obtained in this time was about 80 mg/1, the same as that in batch culture with a flask and spinner flask. We finally obtain 1.5 g of monoclonal antibody per day, and totally 10 g after 14 days of continuous cultivation. In this way, we could easily obtain a large quantity of antibody on a production scale (about 400 g per year).
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