Abstract
The ‘447’ cytoplasmic male sterility (CMS) in Vicia faba is associated with the presence of virus-like particles (VLPs) contained in the cytoplasm of male sterile plants. These VLPs constitute an excellent diagnostic marker for male sterility. Purified VLPs were injected into BALB/c mice and 1 month later, spleen cells were used for cell fusion. Out of the 88 clones obtained, 27 were secreting monoclonal antibodies specific for VLPs. These monoclonal antibodies gave no cross-reaction with fertile plant tissues. One of these antibodies was used in combination with polyclonal antibodies to develop a very specific double-sandwich enzyme linked immunosorbent assay (ELISA) which allows a quantitative determination of VLPs in crude leaf extracts. In addition to being useful for the selection of stable male sterile lines of Vicia faba, these monoclonal antibodies constitute interesting tools for studying and understanding the mechanism of this unusual male sterility.
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