Abstract
Failure of conventional cancer treatments with radio/chemotherapy is generally attributed to tumor resistance which prevents complete remission. The alternative solution to overcome this resistance is the use of natural products mainly derived from plants. Developing these novel strategies aims to increase the sensitivity of cancer cells by means of new tumor cytotoxic agents. Pectin is known as a miracle polymer of natural origin. Although its usage in cancer therapy is starting to be appreciated, its cytotoxic effect on a wide range of cancer cells could be improved. To this end, different physicochemical modifications need to be applied, in order to enhance the pectin effectiveness.The main goal of the present work is to estimate the antiproliferative effect of crude and modified orange pectins on human laryngeal cancer cells (HEp2) using the sulforhodamine cytotoxicity test (SRB). Different modified orange pectins are produced by alkali (pH MP), heat (HMP), and irradiation (IMP) treatments to enhance their antitumor activity. The detection of death type is carried out by means of fluorescence microscopy after labeling the cells with Acridine Orange (AO) and Ethidium Bromide (BET).The results showed a significant decrease in HEp2 cell viability using HMP compared to the other test groups. Fluorescence microscopy analysis indicated an apoptosis cell death induced by HMP on HEp2 cells.
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