Abstract
Twenty-five established human lymphoid cell lines, initiated from peripheral blood leukocytes of normal donors or patients with various hemopoietic diseases, were found to release MIF into their culture fluid. These cells were also characterized by different rosette test procedures and by the presence of immunoglobulins. The percentage of EAC rosette-forming cells ranged from 30 to 90% and immunoglobulin bearing cells from 50 to 80%. No spontaneous rosettes were ever detected. Kinetic studies performed from the onset of the culture have shown that spontaneous MIF production is closely related to the establishment phenomenon. MIF activity detection in established cultures depends on various in vitro parameters including the cell growth pattern and the target cell used for the migration inhibition test. The significance of MIF production by permanent cell lines bearing B surface markers is discussed.
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