Production of medium-chain fatty acids by co-fermentation of antibiotic fermentation residue with fallen Ginkgo leaves

Abstract

The co-fermentation of antibiotic fermentation residues (AFRs) and fallen Ginkgo leaves at C/N ratios of 10–60 was conducted for medium-chain fatty acids (MCFA) production. It was found that a proper C/N ratio could largely promote the MCFA accumulation. Group with C/N ratio of 50 exhibited highest MCFA production of 133.14 mmol C/L, which was 42 %-121 % higher than the other groups. Through the co-fermentation, substrate condition was optimized with rich micro-nutrients in AFRs and abundant polysaccharides in Ginkgo leaves, the hydrolysis of leaves was promoted by the active microbes in AFRs, and the predominance of CE microbes was also stimulated with the dilution of AFRs. The increased C/N ratio significantly affected the SCFA producers like genus Escherichia Shigella and Proteiniphilum, and enriched CE microbes like genus Romboutsia, Eubacterium and Clostridium_sensu_stricto_12. Functional enzymes analysis showed that both reverse β oxidation and fatty acid biosynthesis pathways were strengthened with the increased C/N ratio.