Abstract
Levansucrase (E.C.2.4.1.10) from B. subtilis was used in a synthesis reaction for the production of maltosylfructose using maltose as acceptor and sucrose as the fructosyl donor. The effect of various parameters was studied in order to minimize the hydrolysis of sucrose and the production of levan. A high total solute concentration (240 g/L of sucrose and maltose) reduced hydrolysis to 20%. Further reductions could only be obtained by decreasing the sucrose/maltose concentration ratio. Optimum fructoside production was obtained at around 70% sucrose conversion, as afterwards, the product was hydrolyzed by the enzyme. Under these conditions the yield could be as high as 70-80% with respect to converted sugar or 46% with respect to total sucrose in the reaction. The product was purified and identified as O-α-D-glucopyranosyl-(1-4)-0-α-D-glucopyranosyl-(l-2)-β-fructofuranoside, a non-reducing trisaccharide known as Erlose which occurs naturally in Aphis pomi honeydew.
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