Abstract
This work aimed to evaluate the production of lipolytic complexes, produced by microorganisms isolated from a biological treatment system of effluents from a hotel. To select the best lipolytic microorganism for use in biotechnological processes, we tested 45 bacterial isolates recovered from the raw effluent of the hotel's restaurant waste tank. Lipase production was assayed in culture medium supplemented with olive oil and rhodamine B, incubated at 25 °C and 30 °C for 24 h - 48 h. Results showed 22 isolates lipase producers. All isolates were inoculated on medium without yeast extract to select the ones with highest enzyme yields. Out of these, nine isolates showed high lipase activity. The strain with the larger halo was assayed in submerged culture using an orbital shaker and a bioreactor, with three different substrates (olive oil, grape seed oil, and canola oil). Isolate G40 identified as Acinetobacter baylyi was selected to run the production assays because it showed the best result in the solid medium. In the bioreactor, maximum lipase production was obtained after 12 h of culture with the three substrates evaluated: 0,358 U/mL.min-1 in olive oil, 0,352 U/mL.min-1 with grapeseed oil, and 0,348 U/mL.min-1 with canola oil.
Highlights
The increasing need for industrial enzymes, especially in the environmental area, is driven by the growing necessity for sustainable solutions
With the advancement of research in this area, other lipolytic bacteria were identified such as Acinetobacter and Brevibacterium (Bhosale et al 2016, Ugras and Uzmez 2016, Sharma 2017)
The effluent object of this study presents a large concentration of lipids, making it suitable for the isolation of bacteria producing lipolytic enzymes to be used in industrial biotechnological processes
Summary
The increasing need for industrial enzymes, especially in the environmental area, is driven by the growing necessity for sustainable solutions. Estimates show that the global market for enzymes grows 6-7% per year and that in 2017 it can reach US$ 7 billion. Studies with different microorganisms and substrates for the production of lipases in liquid media, can contribute to find optimal conditions to obtain high yields of enzymes, and reduce production costs on an industrial scale (Ochsenreither 2016, Feitosa et al 2010). There are many lipase-producing bacteria which are commercially used, mainly from the genera Achromobacter, Alcaligenes, Burkholderia, Chromobacterium, and Pseudomonas. With the advancement of research in this area, other lipolytic bacteria were identified such as Acinetobacter and Brevibacterium (Bhosale et al 2016, Ugras and Uzmez 2016, Sharma 2017)
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