Production of lipases by Alternaria sp. (mbl 2810) through optimization of environmental conditions using submerged fermentation technique

Abstract

The present investigation was conducted for the biosynthesis of extracellular and intracellular lipases by Alternaria sp. (MBL 2810) through submerged fermentation technique. To increase the lipases production various parameters were optimized such as nitrogen, carbon sources and additional additives besides the basic cultural conditions e.g., rate of fermentation, volume of fermentation medium, inoculum size and type and initial pH of fermentation medium. The maximum extracellular and intracellular lipases production was achieved, when fermentation was optimized at room temperature for 24 h at the agitation speed of 100 rpm. Other cultural conditions such as inoculum type and inoculum size (4.0 mL) was also optimized, glycine as an additional carbon source and urea as an additional nitrogen source gave the maximum extracellular lipases activity. Maximum production of extracellular lipases (9.04 ± 0.05 a U/mL) was achieved when volume of fermentation was optimized at 25mL and maximized intracellular lipases (10.04 ± 0.13a U/mL) production was achieved when the medium pH was adjusted at 5.