Abstract
Lipase enzymes from microbial sources are currently enormous attention by potential industrial uses. The aim of the study is to investigate some bioparameters affecting lipase production by spectrophotometric method. Extracellular lipase producing organisms isolated from different places of 'ERNAKULAM DIST'(KERALA) and identified as Micrococcus flavus (NYT) by morphological and biochemical characters and 16 rRNA sequencing .Optimization of lipase production and enzyme activity was measured with varying pH (4-9) , incubation temperature (27 0 C & 37 0 C) , growth media incubation time (24hr,48hr,72hr) , reaction mixture time intervals (10, 20 and 30 min ) and substrates as sunflower oil and palm oil. The maximum lipase production by using sunflower oil as substrate was recorded at pH 8, 27 0 C during 48h of culture period and 10 min incubation time and in palm oil higher production was at pH 7, 27 0 C during 48h of culture period and 10 min incubation time.
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