Abstract
The objective of this work is to propose a new route to produce diacylglycerol (DAG) by combining glycerolysis and esterification to convert the formed monoacylglycerol (MAG) into DAG instead of separating it from the product. Linseed oil was used as the source of triacylglycerol (TAG), oleic acid as free fatty acid (FFA), and phospholipase Novozyme 435® as catalyst. Two methodologies were evaluated: simultaneous reactions of glycerolysis and esterification and sequential reactions of glycerolysis followed by esterification. Both the sequential and simultaneous reactions presented an increase in DAG production compared with pure glycerolysis. The best results were obtained for the sequential reaction in the following conditions: (1) glycerolysis step - 57.7 °C, GLY:TAG 1:1 M ratio, 4 % enzyme; (2) esterification step 57.7 °C, FFA:TAG 1:1 M ratio, 4 % enzyme. In these conditions, oil was obtained with 62.05 w% DAG, which represents an increase of 57 % over the concentration of DAG obtained in the pure glycerolysis step.
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