Production of ligninolytic enzymes by solid state fermentation using Pleurotus ostreatus

Abstract

Abstract Solid state fermentation (SSF) stands out in the production of lignocellulolytic and other industrially important enzymes. SSF, an alternative culture method, has several advantages over the conventional submerged ones, like higher yields of enzymes. The production of ligninolytic enzymes, such as laccase (Lac), manganese peroxidase (MnP), lignin peroxidase (LiP) and aryl alcohol oxidase (AAO) by Pleurotus ostreatus (Jacq.) Pleurotus Kumm. (MCC16) was investigated under SSF, which was performed using a support-substrate from potato peel waste (PPW). PPW as dry and fresh was pretreated with base to neutralize organic acids and distilled water. Chemical content of PPW was investigated. Ligninolytic enzyme production patterns were investigated during the growth of the organism for a period of 23 days at 25 °C in the stationary SSF using pretreated PPW. The highest Lac and MnP activities were determined in dry PPW, pretreated with distilled water as 6708.3 ± 75 U/L and 2503.6 ± 50 U/L on day 17, respectively. In addition, amylase and protease enzyme activities were detected under same conditions. According to the results, PPW has a potential as supports for ligninolytic enzyme production by P. ostreatus under SSF.