Abstract
Reciprocal crosses were made to introduce the red flower color of Delphinium cardinale Hook and D. nudicaule Torr. & A. Gray into the dwarf species, D. grandiflorum L., and an ovule culture was applied to keep hybrid embryos from aborting. Interspecific hybrid plants of D. cardinale × D. grandiflorum and D. grandiflorum × D. nudicaule were successfully obtained, and their hybridity was confirmed by isozyme analyses. Morphological studies indicated that these hybrids were almost intermediate between the female and male parents in leaf shape and flower color. Dwarf plants with red flowers were not obtained. All hybrids showed low pollen fertility and failed to yield viable seeds by self- or backcross-pollination using fertile pollen grains of their parents. The applicability of the ovule culture technique for genetic improvement of the genus Delphinium through interspecific hybridization is discussed.
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