Abstract
Two accessions of Lycopersicon peruvianum Mill. (PI270435, PI126443) carrying Mi-2 and Mi-3, respectively, new root-knot nematode resistance genes, were selected as the male parents for crosses with L. esculentum Mill. in order to produce interspecific hybrids. Crossability barriers between these two distantly related species were circumvented by ovule culture. A total of ten interspecific F1 hybrid plants were produced. The hybrid nature of the putative F1 plants was confirmed by a comparison of several morphological characteristics and a PCR-based assay. Eight of ten hybrid plants were backcrossed with L. esculentum to generate a total of 98 BC1 progeny. Two lines were advanced to the BC2 and BC3 levels. Based on these results, ovule culture was found to be an effective method for the production of novel interspecific F1 and BC1 plants.
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