Production of indole-3-acetic acid by Bacillus circulans E9 in a low-cost medium in a bioreactor

Abstract

Bacillus circulans E9 (now known as Niallia circulans) promotes plant growth-producing indole-3-acetic acid (IAA), showing potential for use as a biofertilizer. In this work, the use of a low-cost medium containing industrial substrates, soybean, pea flour, Solulys, Pharmamedia, yeast extract, and sodium chloride (NaCl), was evaluated as a substitute for microbiological Luria Broth (LB) medium for the growth of B.circulans E9 and the production of IAA. In Erlenmeyer flasks with pea fluor medium (PYM), the maximum production of IAA was 7.81 ± 0.16μgmL-1, while in microbiological LB medium, it was 3.73 ± 0.15μgmL-1. In addition, an oxygen transfer rate (OTR) of 1.04kg O2 m-3 d-1 allowed the highest bacterial growth (19.3 ± 2.18 × 1010CFUmL-1) and IAA production (10.7μgmL-1). Consequently, the OTR value from the flask experiments was used to define the conditions for the operation of a 1L stirred tank bioreactor. The growth and IAA production of B.circulans cultured in a bioreactor with PYM medium were higher (8 and 1.6 times, respectively) than those of bacteria cultured in Erlenmeyer flasks. IAA produced in a bioreactor by B.circulans was shown to induce the root system in Arabidopsis thaliana, similar to synthetic IAA. The results of this study demonstrate that PYM medium may be able to be used for the mass production of B.circulans E9 in bioreactors, increasing both bacterial growth and IAA production. This low-cost medium has the potential to be employed to grow other IAA-producing bacterial species.