Abstract
Application of NCS as the serum supplement in media has been shown to be effective for antibody production for the cell lines tested. High levels of antibody titers in the supernatant can be obtained in a fedbatch culture. The secretion rates in the fedbatch cultures are within an order of magnitude of the optimalin vivo secretion rate of 1 μg/106cell·hr. Replacement of FCS by NCS, where possible, should be considered as an economically viable option for serum supplemented large scale cell culture.
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