Production of human and murine eosinophils in vitro and assay for eosinophil differentiation factors.

Abstract

Bone marrow cells from a number of animal species have been used extensively in liquid and semisolid cultures to study hemopoiesis and to produce functional mature cells and factor-dependent cell lines (for review see ref. ). Neutrophils and macrophages are produced without added growth factors from murine long-term bone marrow cultures (), whereas lymphoid cells (,) and megakaryocytes (reviewed in ref. ) can be induced under certain conditions.