Abstract
Hybrid varieties dominate the red beet market. The breeding process necessary to produce these cultivars is very difficult and time consuming. The application of in vitro gynogenesis can reduce the time needed to produce the corresponding homozygous pure lines to a few months. Our research team has developed a method to obtain red beet doubled haploid plants by gynogenesis. The best medium for gynogenesis induction is the B5 medium with the addition of 0.5mg/L IAA, 0.2mg/L BA, and 322mg/L putrescine, whereas the best medium for shoot induction from embryos proved to be the MS medium supplemented with 0.1mg/L NAA, 0.1mg/L BA, and 0.5mg/L putrescine. The shoots obtained were rooted on MS medium containing half the concentration of microelements and 3mg/L NAA, 160mg/L putrescine, and 20g/L sucrose. Ploidy evaluation of gynogenetic plants was performed by flow cytometry and homozygosity or heterozygosity was determined by two isoenzymatic systems: PGI and AAT.
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