Production of granulomatous inflammation in lungs of rat pups and adults by Sephadex beads.

Abstract

Granulomatous inflammation is a process that involves mononuclear leukocytes as well as other inflammatory cells. The heterogeneity of its appearance may be due to the variety of cytokines and chemokines that are involved. In this study, we compared granuloma formation and bronchoalveolar leukocyte differential in the lungs of rats (2- and 8-wk-old) that were treated intravenously with Sephadex beads. In addition, the kinetics of cytokine and chemokine production was determined in these groups. In adults, the beads caused lung granulomas associated with infiltration of eosinophils and neutrophils and increased eosinophil and neutrophil counts in the bronchoalveolar lavage fluid within 16 h. In pups, the granulomas were formed slowly and did not reach the size achieved in adults. Eosinophils and neutrophils were sparsely found in the periphery of the granulomas, even at 32 h. Pups were also unable to respond rapidly to Sephadex bead treatment with eosinophil and neutrophil infiltration in the bronchoalveolar lavage fluid. Tumor necrosis factor-alpha was significantly increased in both groups, but the cytokine was lower in pups than in adults. Interferon-gamma and eotaxin were increased only in adults, and IL-4 and regulated on activation, normal T cell expressed, and secreted was increased only in pups. In conclusion, the i.v. administration of Sephadex beads produced granulomatous inflammation in the lungs of adult rats, but pups were unable to respond as rapidly to the treatment. In addition, the difference in response between the two age groups was associated with the kinetics of cytokine and chemokine production.