Abstract
A galactooligosaccharide (GalOS)-producing yeast, OE-20 was selected from forty seven strains of yeast growing in Korean traditionalMeju (cooked soybean) and the yeast was tentatively identified asKluyveromyces maxianus varlactis by its morphology and fermentation profile. A maximum yield of 25.1%(w/w) GalOS, which corresponds to 25.1 g of GalOS per liter, was obtained from the reaction of 100 g per liter of lactose solution at 30°C, pH 7.0 for 18 h with an intracellular crude β-galactosidase. Glucose and galactose were found to inhibit GalOS formation. The GalOS that were purified by active carbon and celite 545 column chromatography were supplemented in MRS media and a stimulated growth was observed of some intestinal bacteria. In particular the growth rate ofBifidobacterium infantis in the GalOS containing MRS broth increased up to 12.5% compared to that of the MRS-glucose broth during a 48 h incubation period.
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