Abstract

The feasibility of using pastry waste as resource for glucoamylase (GA) production via solid state fermentation (SSF) was studied. The crude GA extract obtained was used for glucose production from mixed food waste. Our results showed that pastry waste could be used as a sole substrate for GA production. A maximal GA activity of 76.1 ± 6.1 U/mL was obtained at Day 10. The optimal pH and reaction temperature for the crude GA extract for hydrolysis were pH 5.5 and 55 °C, respectively. Under this condition, the half-life of the GA extract was 315.0 minutes with a deactivation constant (kd) 2.20 × 10−3 minutes−1. The application of the crude GA extract for mixed food waste hydrolysis and glucose production was successfully demonstrated. Approximately 53 g glucose was recovered from 100 g of mixed food waste in 1 h under the optimal digestion conditions, highlighting the potential of this approach as an alternative strategy for waste management and sustainable production of glucose applicable as carbon source in many biotechnological processes.

Highlights

  • Food waste is a serious global problem, especially in many developed countries

  • To demonstrate the feasibility of pastry waste as the sole substrate for GA production, solid state fermentation (SSF) was conducted with Aspergillus awamori without addition of any other nitrogen or carbon sources

  • 253.7 ± 20.4 U of GA was produced from one gram of pastry waste on dry basis (d.b.); the GA activity of the crude GA extract was 76.1 ± 6.1 U/mL

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Summary

Introduction

Food waste is a serious global problem, especially in many developed countries. In Hong Kong, over 3500 tons of food wastes are generated every day [1]. Landfilling and incineration are the major practices for managing these wastes in many countries. Due to its high contents of carbohydrates and proteins, food wastes may serve as feedstock in biorefineries for production of fungal enzymes, e.g., glucoamylase (GA) and offers an innovative approach to waste management. Starch is usually the major component of mixed food waste from restaurants [4,5,6], application of GA for food waste hydrolysis to recover glucose from starch, may offer a solution for managing food waste and help to save precious resources. Application of the crude GA extract was studied for hydrolysis of mixed food waste collected from a local restaurant to produce high glucose solution

Glucoamylase Production from Pastry Waste
Material Balance for Glucose Production from 1 kg Mixed Food Waste with
Microorganism
Food Wastes Preparation
Solid State Fermentation for Glucoamylase Production
Glucoamylase Extraction
Food Waste Hydrolysis for Glucose Production
Glucoamylase Activity
Conclusions
Conflicts of Interest
Full Text
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