Abstract
Callus has been induced on needle and bud explants from 2, 6 and 26 year old Picea abies (L.) Karst, trees. Seventy seven percent of the needle explants from the youngest material was productive compared to 0.2% for the old tree. New embryogenic sections appeared in the non-embryogenic callus derived from 2, 6 and 26 year old trees after prolonged culture and was promoted by the use of an established embryogenic callus line in a nurse culture system.
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