Production of Double-Stranded RNA Using the Prokaryotic Promoter-Mediated Bidirectional Transcription.

Abstract

Large-scale and cost-less production of double-stranded RNA (dsRNA) is the basis for the widespread application of dsRNA in agriculture. Bidirectional transcription of target sequence in RNase III-deficient Escherichia coli strain HT115 (DE3) is an efficient way to produce large amounts of dsRNA. Here, we present a detailed method for the production of dsRNA by bidirectional transcription in E. coli from vector construction, induction of expression by isopropylthio-β-galactoside (IPTG), and purification of dsRNA from E. coli.