Abstract
Heat treatment was used as a method of improving the health of grape plants from the phytoplasma. The Binder KBWF 240 growth cabinet was used for thermotherapy, in which plants of the Tsitronny Magaracha variety were cultivated in vitro under simulated conditions with a treatment period of 14 days. The results of repeated testing of samples showed the efficacy of treatment of this grape disease: out of 13 samples of the Tsitronny Magaracha variety, only one revealed the latent form of phytoplasma blackening of grapevine wood Bois noir. To improve the health of grape plants obtained in vitro culture, it is advisable to use the developed technological operations with thermotherapy in the growth cabinet, which allows to rid the planting material of the latent form of phytoplasma associated with blackening of grapevine wood Bois noir.
Highlights
Phytoplasmas have spread in many countries, causing significant damage and raising serious concern in vineyards and phytopathological services
The Binder KBWF 240 growth cabinet was used for thermotherapy, where in vitro grape plants of the Tsitronny Magaracha variety were cultivated under simulated conditions with the treatment period of 14 days
The results of preliminary testing of plants in vitro of the Tsitronny Magaracha grape variety sent on examining showed the presence of a latent form of phytoplasma associated with blackening of grapevine wood (Bois noir) in the samples
Summary
Phytoplasmas have spread in many countries, causing significant damage and raising serious concern in vineyards and phytopathological services. In recent years significant advances have been made in the diagnosis, etiology and epidemiology of phytoplasmic diseases [1,2,3,4,5]. Control of the sanitary condition of young plans, elimination of infected plants and treatment of vineyard with insecticides against leafhopper-vectors form the basis of measures to reduce the spread of phytoplasmas. The protection from the disease of Grapevine Yellows (GY) (Grape vine flavescence dorée phytoplasma) consists in chemical treatment against the vector (Scaphoideus titanus Ball.), which is not suitable for control the disease of grapevine black wood (Bois noir) [6]. The prevalence of diseases is facilitated by the propagation of infected plants in grape nurseries. Measures are being taken to develop protocols for the production of phytoplasma-free grape planting material
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