Abstract
Lactic acid is one the most interesting monomer candidates to replace some petroleum- based monomers. The application of conventional poly-lactic acid (PLA) is limited due to insufficient thermal properties. This limitation can be overcome by blending poly-D and poly-L-lactic acid. The main problem is the limited knowledge of D-lactic acid (D-LA) production. Efficient biochemical processes are being developed in order to synthesize D-LA from orange peel waste (OPW). OPW is an interesting renewable raw material for biorefinery processes of biocatalytic, catalytic or thermal nature owing to its low lignin and ash content. Bioprocessing of the pretreated OPW is carried out by enzymatic hydrolysis and fermentation of the released sugars to produce D-LA. Several strains of the species Lactobacillus delbrueckii ssp. bulgaricus have been evaluated for the production of D-LA from OPW hydrolysate using Lactobacillus delbrueckii ssp. delbrueckii CECT 286 as a reference strain since its performance in this kind of substrate have been widely reported in previous studies. Preliminary results show that Lactobacillus delbrueckii ssp. bulgaricus CECT 5037 had the best performance with a yield of 84% w/w for D-LA production and up to 95% (e.e.).
Highlights
Lactic acid is an important chemical and has attracted a great attention due its widespread applications in the food, pharmaceutical, cosmetic, and textile industries
Preliminary results show that Lactobacillus delbrueckii ssp. bulgaricus CECT 5037 had the best performance with a yield of 84% w/w for D-lactic acid (D-LA) production and up to 95% (e.e.)
These results indicate that orange peel waste (OPW) hydrolysate is an interesting feedstock for the production of D-LA, since the product yield is close to its theoretical value (1 g g-1 ) in most cases
Summary
Lactic acid is an important chemical and has attracted a great attention due its widespread applications in the food, pharmaceutical, cosmetic, and textile industries. Polylactic acid (PLA) is a biodegradable polymer with great potential in replacing petrochemical polymers and L-and. D-lactic acids are prominent monomers of the bioplastic industry [1]. D-lactic acid monomers or oligomers [2,3,4,5,6]. Microbial production of optically pure lactic acid has extensively been studied because chemically synthesized lactic acid is a racemic mixture [7]. The L-isomer has been studied in detail, information on biosynthesis of D-lactic acid (D-LA) is still limited [5,9]
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