Abstract
A fungal strain (Aspergillus sp.S6) isolated from soil and identified as Aspergillus aculeatus was found to produce xylanase using SSF. The optimum conditions for enzyme – substrate reaction were pH: 4.8, temperature: 60°C, incubation time: 5 min, substrate concentration: 5mg/ml. Enzyme activity was stimulated by Ca2+, K+ and Cu2+ while completely inhibited by Hg2+ and Ag+. Untreated moistened wheat bran at 25°C subjected to 72 h fermentation using Aspergillus aculeatus provided maximum xylanase production (253.98IU/gds). Supplementation with xylan (1%) and ammonium chloride (0.5 – 1%) improved enzyme production. Small catabolic repression occurred in xylose and glucose supplemented wheat bran.
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