Abstract
BackgroundPakistan is one of the top five date fruit-producing countries and produced more than 30% wastes in picking, packing, storage, and commercialization stages. The date fruit wastes are usually considered inedible for humans and only used for livestock feed. In current research, Bacillus licheniformis KIBGE-IB3 was screened for pectinase, xylanase, cellulase, and amylase production using date fruit wastes as substrate through solid state fermentation. ResultsThe B. licheniformis KIBGE-IB3 produced higher concentration of pectinase using date fruit wastes as substrate as compared to amylase, cellulase, and xylanase. B. licheniformis KIBGE-IB3 produced maximum pectinase using 5.0 g/dl date fruit wastes and 0.5 g/dl yeast extract. B. licheniformis KIBGE-IB3 required pH 7.0, 37 °C incubation temperature, and 72 h incubation period for maximum production of pectinase. ConclusionIt has been concluded that date fruit waste is a good source of biomass and can be utilized for the commercial production of pectinase.
Highlights
Using of date fruit wastes for the production of enzymes Bacillus licheniformis Khan Institute of Biotechnology and Genetic Engineering (KIBGE)-IB3 were inoculated in four different media specific for each enzyme production with the addition of 5% date fruit wastes in each medium
It was observed that Bacillus licheniformis KIBGE-IB3 produced pectinase, xylanase, amylase, and cellulase using date fruit wastes but pectinase production was higher as compared to the others enzymes (Table 2)
In the current study, Bacillus licheniformis KIBGE-IB3 was screened for the synthesis of amylase, xylanase, cellulase, and pectinase using date fruit wastes as substrate by solid state fermentation
Summary
Pakistan is one of the top five date fruit-producing countries and produced more than 30% wastes in picking, packing, storage, and commercialization stages. The date fruit wastes are usually considered inedible for humans and only used for livestock feed. Bacillus licheniformis KIBGE-IB3 was screened for pectinase, xylanase, cellulase, and amylase production using date fruit wastes as substrate through solid state fermentation. More than 4000 enzymes are identified and 200 are used for industrial preparations. Microbial enzymes are usually preferred for commercial applications due to technical easiness to obtain, economical feasibility, and easy product recovery and optimization. The cost of microbial enzymes depend on the substrates used to induce the enzyme production during fermentation [1]. B. subtilis, B. stearothermophillus, B. licheniformis, and B. amyloliquefaciens are reported extensively as industrial
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