Production of collagen by cells isolated from a retrocorneal fibrous membrane rabbit model

Abstract

The morphological and biosynthetic characteristics of cells from an experimentally induced rabbit retrocorneal fibrous membrane (RCFM) model were investigated. By transmission electron microscopy the cells within the RCFM demonstrated overlapping cytoplasmic processes and intercellular junctions, neither of which are fibroblast-like characteristics. The extracellular matrix within the RCFM had a fibrillar and amorphous component. Collagenous biosynthetic products of primary cultures of RCFM cells were compared to normal corneal endothelial cells, which produce mainly type IV collagen and a small amount of type V collagen, and fibroblasts, which produce types I, III and V collagens. The collagenous components produced by the RCFM cells were a combination of types I, V and low molecular weight fragments of type IV collagen. Therefore, these morphological and biochemical data suggest that RCFM cells are a type of modified corneal endothelial cell that produce collagens distinctly different from normal corneal endothelial cells.