Abstract
A cloned epithelial cell line of rat liver origin produced collagen in vitro. The amount of collagen in the cell layers increased linearly with time after the cultures reached a saturation density. Two kinds of acidic glycosaminoglycans, hyaluronic acid and dermatan sulfate, were detected from the same cell layers by the combined method of electrophoresis and enzymic hydrolysis. Quantitative analysis by densitometry with an electropherogram showed that a rapid increase of both sulfated and non-sulfated glycosaminoglycans occurred concurrently with an accumulation of collagen. Histochemical stainings revealed that the majority of Alcian blue-positive materials was located outside the cells, where argyrophilic fibers could be found. Our data are consistent with the results obtained from biochemical experiments on cirrhotic rat livers, suggesting that liver parenchymal cells are responsible for an accelerated production of both collagen and glycosaminoglycans in liver cirrhosis.
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