Production of Chitinase and its Optimization from a Novel Isolate Serratia marcescens XJ-01

Abstract

Production of chitinase from bacteria has distinct advantages over fungi, due to the formation of mycelia of fungi in the later phase of fermentation. A novel chitinase-producing bacterial strain XJ-01 was isolated from the Yulu fishing field of Changsha, Hunan province, China, by enrichment and spread-plate technique, sequentially. Physicochemical characterization and 16S rRNA sequencing revealed that strain XJ-01 belongs to Serratia marcescens. By optimizing the fermentation condition based on L(9)(3(4)) orthogonal experimental design, a maximal chitinase activity up to 15.36U/ml was attained by that stain under the condition: 0.5% (NH(4))(2)SO(4) as the nitrogen source, 0.75% colloidal chitin as the carbon source, temperature of 32°C, time of 32h and pH 8.0.