Abstract
Optimization of cellulase production by Myceliophthora thermophila BJTLRMDU3 was studied in solid state fermentation. Sequential use of Plackett-Burman and response surface methodology (RSM) resulted in the production of 98.81, 243.19, and 316.48 U/g dry moldy residue (DMR) of FPase, CMCase, and β-glucosidase, respectively. Statistical optimization has resulted in more than 4.0-fold increase in the production of cellulases. Optimization of saccharification of untreated and pretreated rice straw was carried out by cellulolytic enzymes of thermophilic mold M. thermophila. The liberation of reducing sugars was higher using fungal cellulases in ammonia-pretreated rice straw as compared with untreated biomass. Maximum liberation of reducing sugars was attained at 60 °C (224.24 mg/g substrate) and pH 5.0 (246.33 mg/g substrate) after an incubation time of 24 h (345.61 mg/g substrate) using enzyme dose of 20 U/g in ammonia-pretreated rice straw. Supplementation of xylanase further enhanced the saccharification (488.78 mg/g substrate) of pretreated rice straw. Analysis using high-performance liquid chromatography (HPLC) indicated the presence of various monomeric and oligomeric sugars in the enzymatic hydrolysate of pretreated rice straw. Both, Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) revealed the structural changes in rice straw after ammonia pretreatment.
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