Abstract
The present study aims at isolating, screening, characterizing and optimizing cellulolytic bacteria isolated from soil samples. Different soil samples were collected from different locations of district Jaunpur, Uttar Pradesh, India. The morphological, biochemical and molecular characterization of the isolates were carried out for their identification. A total number of 23 isolates were screened at preliminary level for cellulolytic activity out of which three potent cellulase producers were selected for further study. On the basis of 16S rDNA sequencing these three isolates were identified as Bacillus velezensis PS-14, Bacillus velezensis PS-09 and Bacillus paramycoides PS-05. The optimal conditions for cellulase enzyme production was also determined and maximum activity was obtained at pH 7 and 40 0 C temperature, while 2% inoculum level and 48 hr incubation period were found to be optimum for cellulase production by all the strains. Glucose and Carboxymethyl cellulose was proved to be the best carbon sources, while ammonium choride and peptone was observed to be the most favourable nitrogen source for all three bacterial isolates.
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