Abstract
A new strain of the yeast Metschnikowia koreensis was grown in shake flasks and a stirred bioreactor for the production of carbonyl reductase. The optimal conditions in the bioreactor for maximizing the biomass specific activity of the enzyme were found to be: a medium composed of glucose (20g/L), peptone (5g/L), yeast extract (5g/L) and zinc sulfate (0.3g/L); the pH controlled at 7; the temperature controlled at 25°C; an agitation speed of 500rpm; and an aeration rate of 0.25vvm. In the bioreactor, a biomass specific enzyme activity of 115.6U/gDCW was obtained and the maximum biomass concentration was 15.3gDCW/L. The biomass specific enzyme activity obtained in the optimized bioreactor culture was 11-fold higher than the best result achieved in shake flasks. The bioreactor culture afforded a 2.7-fold higher biomass concentration than could be attained in shake flasks.
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