Abstract
BackgroundBP178 peptide is a synthetic BP100-magainin derivative possessing strong inhibitory activity against plant pathogenic bacteria, offering a great potential for future applications in plant protection and other fields. Here we report the production and recovery of a bioactive BP178 peptide using rice seeds as biofactories.ResultsA synthetic gene encoding the BP178 peptide was prepared and introduced in rice plants. The gene was efficiently expressed in transgenic rice under the control of an endosperm-specific promoter. Among the three endosperm-specific rice promoters (Glutelin B1, Glutelin B4 or Globulin 1), best results were obtained when using the Globulin 1 promoter. The BP178 peptide accumulated in the seed endosperm and was easily recovered from rice seeds using a simple procedure with a yield of 21 μg/g. The transgene was stably inherited for at least three generations, and peptide accumulation remained stable during long term storage of transgenic seeds. The purified peptide showed in vitro activity against the bacterial plant pathogen Dickeya sp., the causal agent of the dark brown sheath rot of rice. Seedlings of transgenic events showed enhanced resistance to the fungal pathogen Fusarium verticillioides, supporting that the in planta produced peptide was biologically active.ConclusionsThe strategy developed in this work for the sustainable production of BP178 peptide using rice seeds as biofactories represents a promising system for future production of peptides for plant protection and possibly in other fields.
Highlights
BP178 peptide is a synthetic BP100-magainin derivative possessing strong inhibitory activity against plant pathogenic bacteria, offering a great potential for future applications in plant protection and other fields
Based on the library of BP134 derivatives [52], in which modifications in peptide sequences were introduced to facilitate their production in plant systems, we selected the BP178 peptide for its production in rice seeds
Such evidences include the accumulation of a similar protein peptide (Cec A) to protein body (PB) when identical regulatory sequences were utilized for cloning and transformation, and the presence of BP178 in cell fractions that have been shown to be enriched for protein bodies [53, 54]
Summary
BP178 peptide is a synthetic BP100-magainin derivative possessing strong inhibitory activity against plant pathogenic bacteria, offering a great potential for future applications in plant protection and other fields. The rational design of AMPs may overcome these limitations by developing peptides with improved biological properties, like increased antimicrobial activity and stability, and reduced toxicity against non-targeted organisms [7,8,9]. In this context, a library of synthetic linear undecapeptides consisting of cecropin A (1–7)melittin (2–9) hybrids (named CECMEL11 library), was previously obtained by combinatorial chemistry [8].
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