Abstract
A group of biosurfactants are lipopeptides that are produced by some microorganisms, especially Bacillus strains. They are new bioactive agents with anticancer, antibacterial, antifungal, and antiviral activities. Also, they are used in sanitation industries. In this study, a lead-resistant strain of Bacillus halotolerans was isolated for lipopeptide production. This isolate exhibited metal resistance (lead, calcium, chromium, nickel, copper, manganese, and mercury), salt tolerance (12%), and antimicrobial activities against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Saccharomyces cerevisiae. The production of lipopeptide was optimized, concentrated, and then extracted from the polyacrylamide gel in a simple way for the first time. The nature of the purified lipopeptide was determined by FTIR, GC/MS, and HPLC analyses. The purified lipopeptide indicated significant antioxidant properties (90.38% at a concentration of 0.8mgml-1). Also, it had anticancer activity by apoptosis (flow cytometry analysis) in MCF-7 cells, while it had no cytotoxicity on HEK-293 normal cells. Therefore, Bacillus halotolerans lipopeptide has the potential to be used as an antioxidant, antimicrobial, or anticancer agent in the medical and food industries.
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