Abstract
Bioethanol production has been a challenge for the researchers with respect to enhancing the bioethanol yield. In this study, we are reporting an efficient novel method to produce bioethanol. The process comprises co-culture technique to produce bioethanol from wheat straw, by co-culturing Bacillus licheniformis and Saccharomyces cerevisiae. Simultaneous saccharification and fermentation allows wheat straw hydrolysis by cellulase enzyme produced by Bacillus licheniformis and conversion of produced reducing sugar into ethanol by Saccharomyces cerevisiae. Pre-treatment of wheat straw and optimization of co-culturing parameters like, time, pH, substrate concentrations and nitrogen source concentrations gave a net yield of ⁓ 4.11 g/l bioethanol. Scale up of optimised media to fermenter has resulted in a significant enhancement of bioethanol production to ⁓ 14.70 g/l.
Highlights
IntroductionWorld’s requirement for fuel is increasing at high rate in contrast to the fossil fuels [1]
In the present state, world’s requirement for fuel is increasing at high rate in contrast to the fossil fuels [1]
Production of fuel from waste products has the potential to provide a solution for cost effectiveness and sustainability
Summary
World’s requirement for fuel is increasing at high rate in contrast to the fossil fuels [1]. Ethanol is an eco-friendly fuel in contrast to other substrates which causes green-house gas emissions when employed as fuel [2]. It is being used as an additive to biodiesel and have increased the biodiesel’s energy yield for automobile industry [3]. There is need for a cost effective and profuse source of ethanol [6]. A process is required which can convert substrate into ethanol with less complexity and cost effectiveness
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