Abstract
Abstract Processing (milling, oil extraction) of agricultural products result in accumulation of massive amount of agro-waste residues. A sustainable alternative technique is required to utilize those agro-waste residues through biotechnology to convert them into useful products. α-Amylase is an enzyme of glycoside hydrolases family which hydrolyzes the α-D-(1,4) glycosidic bond. In the present research, Bacillus tequilensis TB5 was hired for the α-amylase production through SmF by utilizing agro-waste substrate rice bran. Also, the role of varying Physico-chemical parameters on α-amylase production was evaluated to determine the optimal conditions required for its maximum production. The findings of this research revealed that the optimal conditions for maximum yield (39.736 ± 0.296 U/ml) were found at pH 6.0, temperature 37 °C and incubation period of 72 h. On analyzing influence of various nutritional supplement on enzyme production, it was found that some of the nutrients like; peptone, beef extract, ammonium chloride, ammonium sulphate can enhance enzyme yield at a particular concentration. Purification of α-amylase was also done through ammonium sulphate precipitation method and then molecular weight of 54 kDa was determined by SDS-PAGE. The present research carried strongly supports, that rice bran is an efficient agro-waste substrate can possibilities of the commercial production of α-amylase.
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