Abstract
Lyophilized bacteria lose their ability to form colonies on nutrient media upon exposure to air (Naylor & Smith, 1946; Lion & Bergmann, 1961), oxygen being the lethal factor (Lion & Bergmann, 1961; Lion, 1963). The ability to reproduce decreases with exposure time. The ‘death’ of freeze-dried bacteria exposed to oxygen (FDO bacteria) does not abolish their ability to synthesize DNA, RNA and protein (Novick, Israeli & Kohn, 1972). However, DNA synthesis ceases a short time after reconstitution and β-galactosidase production is blocked, to be resumed only after a repair period. In order to check whether protein and DNA synthetic mechanisms could function correctly under some other control than that of the cell, we investigated the ability of FDO bacteria to produce phage.
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