Abstract
Monensin is converted to monensin bromoacetate, which provides successful coupling to protein and allows production of monensin-specific rabbit antisera. A modified indirect enzyme-linked immunosorbent assay (ELISA) was developed, which is highly sensitive (2 ng/mL) to monensin determination. Monensin recovery was 53-81% at 10 ppb in sera or urine, and 81-130% at 100 ppb in dichloromethane-extracted feces or water. Overall recovery was 98.8% with coefficients of variation from 6 to 52% over the range of monensin concentrations studied. This is the first immunoassay reported for the carboxylic ionophore antibiotics.
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