Abstract
Microbial biotransformation is a great model system to produce drugs and biologically active compounds. In this study, we elucidated the fermentation and production of an anti-cancer agent from a microbial process for regiospecific hydroxylation of resveratrol. Among the strains examined, a potent strain showed high regiospecific hydroxylation activity to produce piceatannol. In a 5 L (w/v 3 L) jar fermentation, this wild type Streptomyces sp. in the batch system produced 205 mg of piceatannol (i.e., 60% yields) from 342 mg of resveratrol in 20 h. Using the product, an in vitro anti-cancer study was performed against a human cancer cell line (HeLa). It showed that the biotransformed piceatannol possessed a significant anticancer activity. This result demonstrates that a biotransformation screening method might be of therapeutic interest with respect to the identification of anti-cancer drugs.
Highlights
IntroductionResveratrol (3,4,5-transhydroxystilbene), one of the most widely studied polyphenols produced by plants, is of growing scientific interest, since it enhances the health-related qualities in humans [1,2,3]
Resveratrol (3,4,5-transhydroxystilbene), one of the most widely studied polyphenols produced by plants, is of growing scientific interest, since it enhances the health-related qualities in humans [1,2,3].It is well-known for its presence in red wine
It is a significant fact that the screening of a wide variety of microorganism living in environments is an efficient approach to pave the way for novel drugs and bioactive compounds
Summary
Resveratrol (3,4,5-transhydroxystilbene), one of the most widely studied polyphenols produced by plants, is of growing scientific interest, since it enhances the health-related qualities in humans [1,2,3]. It is well-known for its presence in red wine. The health effect of red wine is believed to result from its high concentration of polyphenols, including resveratrol [3,4,5]. Regiospecific hydroxylation of aromatic compounds by chemical synthesis is difficult and involves diverse reaction steps. Regiospecific microbial hydroxylation at a non-activated carbon atom of aromatic compounds is attractive and remarkable biosynthesis. To the best of our knowledge, this is first report to show the production of piceatannol using microbial biotransformation
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
