Abstract
A high quality protein isolate has been obtained from defatted sunflower meal by alkaline extraction and isoelectric precipitation. Protein content was increased from 31.2 % in the defatted flour to 97 % in the protein isolate. The percentages of fiber, soluble sugars, polyphenols and residual lipids in the protein isolate were reduced to more than 90 % with respect to the defatted meal. The protein isolate was used as starting material for the generation of an extensive enzymatic protein hydrolysate. The hydrolysis was carried out in a pH stat using sequentially an endo-protease (Alcalase) and an exo-protease (Flavourzyme). The protein hydrolysate, with a degree of hydrolysis of 50.7 %, was white and non bitter.
Highlights
The increasing growth of population and the reduction in food resources in the third world and the excessive intake of animal proteins which are unhealthy for the ingestion of the associated saturated fats, have led to interest in the search for new sources of proteins from non-conventional raw materials.Sunflower is one of the most important oilseed crops cultivated in the world, ranking fourth with respect to oil production
Plant protein isolates constitute an optimal material for the supplementation of new types of foods and are a good substrate for the generation of enzymatic protein hydrolysates
Protein isolates are enriched in total proteins and have low amounts of compounds such as soluble sugars, lipids, phenols or fiber
Summary
Sunflower is one of the most important oilseed crops cultivated in the world, ranking fourth with respect to oil production. Defatted meal is the main by-product after oil extraction. This meal, containing about 40-45 % protein, may constitute an optimal source of proteins for humans. To make use of these proteins, sunflower meal requires processing to lower the contents in undesirable components such as soluble sugars, polyphenols and lipids that may reduce the chemical and nutritive value of the proteins. Fiber, one of the main components of the defatted meal, should be eliminated to enrich the final product in protein
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