Abstract
Fusarium roseum (Lk.) Snyd. and Hans. ‘Avenaceum’, which causes a dry rot of Solanum tuberosum L., produces an extracellular endo-polygalacturonate trans-eliminase when cultured on a potato broth-pectic acid medium or on intact potato tubers. This enzyme is calcium dependent and exhibits maximum activity between pH 9·0 and 9·5. It has been purified 117-fold by ammonium sulfate fractionation and electrofocusing (pH range 7 to 10), and behaves as a single component with an isoelectric point of pH 8·2. It has a molecular weight of c. 30·5 × 10 3 as determined by sucrose density gradient centrifugation and gel filtration in Sephadex G-75. Both crude and purified preparations of the enzyme cause maceration and cell death of potato tuber tissue.
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