Abstract
A YY-female Poecilia reticulata was produced by endocrine sex reversal followed by progeny testing. Heterogametic females (XY) produced by endocrine sex reversal were mated with normal males (XY) and subsequently treated with an estrogen-supplemented diet, 5–10 days prior to parturition. Progenies obtained from these fish were individually mated with sex-reversed males (XX) to identify their genotype. A single female was identified to have the YY-genotype, and produced only males when mated with a sex-reversed male (XX).
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