Production of a recombinant lipase artificially localized on the Bacillus subtilis cell surface

Abstract

The lipB gene encoding an extracellular lipase, LipB, from Bacillus subtilis was fused to the 5′-terminal region of the cwlB gene encoding the cell wall-binding domain of the major cell wall hydrolase (autolysin). SDS-polyacrylamide gel electrophoresis and zymography revealed that the fused protein is localized in the B. subtilis cell wall fraction and retains lipase activity.