Abstract
Productivity levels were analysed at a pilot-scale version of Hollow Fiber (HF) system Cell-Pharm 2500 (35 ft2)/THP-1/1% FCS/500 μM ferric citrate medium. We evaluated both expanded bed adsorption (EBA) and conventional packed bed (PB) (Protein A) to purify monoclonal antibody (Mab) IgG2b from feedstocks. Direct capture by STREAMLINE EBA resulted in 92% product recovery and 7-fold more concentrated product. Process time and buffer consumption were also improved under EBA method (reduced). The purity (measured by amount of host cell and medium proteins, DNA and SDS-PAGE) of eluted Mab by EBA was comparable to the purity obtained on the standard packed method using Protein A media.
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