Abstract

Cassava plays a key role in the food production and economies of several countries worldwide. Due to its starch content, alcoholic fermentation is a promising transformation process for adding value to cassava. However, most of the existing cassava beverages are from traditional origin, with the yields and quality often poorly known or controlled due to the use of artisanal production processes. This work aims at the application of easily implementable biotechnological tools for the production of cassava spirits, in order to add value to this raw material. Cassava flour was liquefied and saccharified using enzymatic cocktails, generating a fermentable broth with ~184 g L−1 of fermentable sugars. This was then fermented into an alcoholic product with ~10% ethanol by volume and distilled for spirit production. Cassava spirits with 40% ethanol by volume, with or without application of oak wood, were produced. For further valorization, volatile fractions of cassava spirits were characterized by gas chromatography–flame ionization detection (GC-FID) and GC–MS. These showed a predominance of yeast fermentation metabolites, complemented by wood extractives where oak chips were applied. Both produced spirits showed desirable sensory traits, receiving good acceptance by experienced tasters, demonstrating the feasibility of the proposed process to add value to cassava surplus.

Highlights

  • Cassava (Manihot esculenta) is a prominent food crop, widely known for feeding a significant fraction of the world’s population

  • Cassava can be used as a raw material for different purposes: it can be directly used as a food source, serve as livestock feed, or be transformed for production of energy or other commodities [3,4]

  • Cassava does not have the hydrolytic enzymatic activity typically found in malt, being that specific enzymes had to be supplemented for liquefaction and saccharification, mimicking a mashing step

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Summary

Introduction

Cassava (Manihot esculenta) is a prominent food crop, widely known for feeding a significant fraction of the world’s population. This tuberous root, which is an excellent source of starch, grows on a wide variety of soils, and it is currently cultivated throughout tropical and subtropical regions worldwide [1]. By 2014, cassava production occurred in 103 countries, accounting for 270 million tons, and 25 million hectares cultivated worldwide, making it the 5th most prominent staple crop [2]. When cassava surplus is available, Molecules 2020, 25, 3228; doi:10.3390/molecules25143228 www.mdpi.com/journal/molecules

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