Abstract
The influence of working conditions on the growth batch of Pseudomonas putida CECT5279 has been studied, in regard to both the growth rate and the desulfurization capability accumulated in the cells. These operational conditions include pH conditions (buffered and nonbuffered media, using different carbon sources (glucose, citrate, and glutamic acid)), operating temperatures (26−32 °C), and different dissolved oxygen concentrations, due to different aeration conditions (different air flows, using enriched air, etc.). Pseudomonas putida CECT5979, which is a genetically modified microorganism (GMO), has the ability to convert dibenzothiophene (DBT) to 2-hydroxybiphenyl (HBP), desulfurizing the organic molecule. To get the best conditions to obtain desulfurizing cells, a parameter (DBDS) that incorporates both biomass concentration and time to reach a particular percentage of desulfurizing capability (XBDS) has been used. The optimum value of DBDS has been obtained under the following working conditions: te...
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
