Abstract
The production conditions of 3-hydroxybutyrate (3-HB) monomers in submerged culture using Pseudomonas mendocina DS04-T as a degrading strain were optimized. The optimal culture medium constituents (w/v) were determined as follows: 0.5 % PHB, 0.15 % NH4Cl, 1.2 % Na2HPO4·12H2O, 0.3 % KH2PO4, 0.05 % MgSO4·7H2O, and 0.0005 % CaCl2·2H2O. The optimum parameters for liquid fermentation were as follows: temperature, 30 °C; inoculum content, 1.0 %; cultivation time, 18 h; initial pH, 7.0; volume of medium, 100 mL; and rotary speed, 180 rpm. Yield of 3-HB monomer and PHB depolymerase activity at optimized conditions were (56.4 ± 0.8) % and (57.4 ± 2.6) U/mL, respectively. The 3-HB monomer concentration obtained under optimized conditions was 1.5 times that obtained under the basic culture medium and initial conditions.
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